Mitigation of Diclofenac-Induced Hepatorenal Toxicity by Methanolic Extract of Cymbopogon citratus

Authors

DOI:

https://doi.org/10.54548/njps.v39i2.10

Abstract

Despite the wide range of advantages of non-steroidal anti-inflammatory drugs (NSAIDs) such as diclofenac, their abuse can have a deleterious impact on the hepatorenal system. This study evaluated the histopathological and molecular effects of NSAIDs-induced hepatorenal toxicity on the expression of TGFβ and Nrf2 in the liver and kidneys of adult male Wistar rats treated with methanolic leaf extracts of Cymbopogon citratus (lemon grass). Twenty adult male Wistar rats were randomly divided into four groups. The first group were the unexposed control rats administered with distilled water only, while the test groups (II-IV) were orally administered with diclofenac at a standard dosage of 5mg/kg/BW. The rats in groups III and IV were administered with methanolic extract of Cymbopogon citratus at 100mg/kg/BW and 200mg/kg/BW respectively. After 28 days, the rats were euthanized by cervical dislocation, and the liver and kidneys were histologically processed. mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf-2) and transforming growth factor beta (TGF-β) was then analyzed. The results revealed significant pathological alterations in the histoarchitecture of the livers and kidneys of the untreated NSAIDs-exposed rats with the administration of Cymbopogon citratus effectively reducing oxidative damage by modulating the expression of Nrf-2 and TGF-β while also ameliorating the histological derangement observed in the studied organs. Cymbopogon citratus modulates the expression of TGF-β and Nrf-2 in the liver and kidneys of the experimental animals consequently mitigating diclofenac-induced oxidative damage in the studied organs.

Author Biographies

  • Temidayo Adeniyi, University of Ilorin, Kwara State Nigeria.

    Senior lecturer

    Department of Medical Laboratory Science, College of Health Sciences,

    University of Ilorin, Kwara State Nigeria.

    adeniyi.td@unilorin.edu.ng

  • Akinpelu Moronkeji, University of Medical Sciences Ondo, Nigeria

    Lecturer I,

    Department of Medical Laboratory Science,

    University of Medical Science Ondo-City,

    Ondo State, Nigeria.

    amoronkeji@unimed.edu.ng

  • Olayinka Ajala

    General Medicine Department,

    Kent and Canterbury Hospital,

    United Kingdom.

  • Abiodun Oyeleke, Federal University Oye-Ekiti, Ekiti State.

    Lecturer I

    Department of Anatomy,

    Federal University Oye-Ekiti,

    Ekiti State.

    abiodun.oyeleke@fuoye.edu.ng

  • Adebimpe I Moronkeji, Elizade University Ilara Mokin

    LecturerII,

    Department of Medical Laboratory Science,

    Elizade University Ilara Mokin, Ondo State,

    Nigeria.

  • Favour Falana , University of Medical Sciences Ondo

    Medical Laboratory Scientist,

    Department of Medical Laboratory Science,

    University of Medical Sciences Ondo,

    Nigeria.

  • Ngeri Belema Belema

    Department of Medical Laboratory Science,

    Faculty of Allied Health Sciences,

    University of Medical Sciences,

    PMB 536,

    Ondo City, Ondo State,

    Nigeria. 

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2024-12-31

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Mitigation of Diclofenac-Induced Hepatorenal Toxicity by Methanolic Extract of Cymbopogon citratus. (2024). Nigerian Journal of Physiological Sciences, 39(2), 241-249. https://doi.org/10.54548/njps.v39i2.10