Deteriorating Hemostatic Functions of Adult Female Wistar Rats Mediated by Activities of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) – Piroxicam and Vitamin E
Słowa kluczowe:
Hemostasis, NSAIDs, Piroxicam, Bleeding Time, Clotting Time, FibrinogenAbstrakt
Summary: The status of hemostatic parameters, are useful physiological markers of organ and tissue damage and dysfunction. This study investigated the effect of Piroxicam on some hemostatic parameters of albino Wistar rats. Twenty-four (24) female albino Wistar rats were used for this study, they were randomly divided into four (4) groups of six (6) rats each. Group A served as control, Group B and C were rats treated with 0.1 mg/kg and 0.2 mg/kg piroxicam while Group D served as 0.2 mg/kg piroxicam treated rats administered with Vitamin E. The experiment lasted for a period of 4 weeks, after which the rats were euthanized. Blood sample was collected for measurement of bleeding time, clotting time, fibrinogen level and platelets count. One-way ANOVA was used to compare, means and a p<0.05 was considered significant. Result: Data generated showed that Piroxicam significantly (p<0.05) decreased the clotting time, platelets count and fibrinogen level. Piroxicam also significantly (p<0.05) increased the bleeding time level of the rats. Co-administration of Vitamin E significantly (p<0.05) increased the bleeding time, it also significantly decreased the clotting time, fibrinogen level and platelets counts. Conclusion: This study therefore shows that Piroxicam impairs hemostasis while Vitamin E administration further enhances the activities of Piroxicam on hemostatic parameters.
Bibliografia
Barroso, A.B., Lima, V., Guzzo, G.C., Moraes, R.A., Vasconcellos, M.C. and Bezerra, M.M. (2006). Efficacy and safety of combined piroxicam, dexamethasone, orphenadrine, and cyanocobalamin treatment in mandibular molar surgery. Braz J Med Biol Res. 39: 1241- 1247
Bharadwaj JR. Laboratory investigation of haemolytic and purpuric disorders. In: Laboratory manual of the armed forces 2001;1:41-54.
Conrad, K.A., Fagan, T.C., Mackie, M.J. and Mayshar, P.V. (2008). Effects of ketorolac tromethamine on haemostasis in volunteers. Clin Pharmacal Ther. 43:542-546
Cox, S.R., VanderLugt, J.T., Gumbleton, T.J., Smith, R.B. (2007). Relationships between thromboxane production, platelet aggregability, and serum concentrations of ibuprofen and flurbiprofen. Clin Pharmacal Ther. 41:510-521.
Cronberg, S., Wallmark, E. and Soderberg, I. (2004). Effect on platelet aggregation of oral administration of 10 non-steroidal analgesics to humans. Scand 1 Haematol 33:155-159
Danesh, J., Lewington, S. and Thompson, S.G. (2005).. Plasma fibrinogen level and the risk of major cardiovascular diseases and nonvascular mortality: and individual participant meta-analysis. JAMA. 294:2848.
Dowd, P. and Zheng, Z.B. (1995). On the mechanism of the anticlotting action of vitamin E quinone. Proc Natl Acad Sci USA 92:8171–5.
Franchini, M., Capra, F., Targher, G., Montagnana, M. and Lippi, G. (2007). Relationship between ABO blood group and von Willebrand factor levels: from biology to clinical implications. Thrombosis Journal., 5: 14
Freedman, J. E., Farhat, J. H., Loscalzo, J. and Keaney, J. F., Jr. (1996) a-Tocopherol inhibits aggregation of human platelets by a protein kinase Cdependent mechanism. Circul. 94: 2434–2440
Graziani, F., Corsi, L., Fornai, M., Antonioli, L., Tonelli, M. and Cei, S. (2005). Clinical evaluation of piroxicam-FDDF and azithromycin in the prevention of complications associated with impacted lower third molar extraction. Pharmacol Res. 52: 485-490.
Green, D., Davies, R.O., Holmes, G., Kohl, H., Lee, R.B., Reynolds, N., Schmid, F.R. and Tsao, C. (2001). Effects of diflunisal on platelet function and fecal blood loss. Clin Pharmacal Ther.30:378-384.
Ha, H., Yu M.R. and Kim, K.H. (1999). Melatonin and taurine reduce early glomerulopathy in diabetic rats. Free Radic. Biol. Med., 26: 944-950.
Handoll H.H.G., Farrar M.J., McBirnie J., Tytherleigh-Strong G.M., Milne A.A. and Gillespie W.J. Heparin, low molecular weight heparin and physical methods for preventing deep vein thrombosis and pulmonary embolism following surgery for hip fractures. Cochrane Database Syst. Rev. 2002; 2: 305-305
Harris, D.T., Gilding H.P. and Smart, W.A.M. (1956). Experimental Physiology for Medical Students. 6th Edn., Sagar Publications, New Delhi.
Ingram, G.I. (1961). A suggested schedule for the rapid determination of acute haemostatic failure. J. Clin. Pathol., 14: 356-360.
Issitt, P.D. (1985). Applied blood group serology, Montgomery Scientific Publications.
Kaur M, Singh A, Bassi R, Kaur D. (2015). Blood group distribution and its relationship with bleeding time and clotting time. Natl J Physiol Pharm Pharmacol. 5.253-257
Klaassen, C. D. (2001). Casarett and Doulls Toxicology: the Basic Science of Poison. 6th Eds The McGrau-Hill Companies Inc. New York
Laffan, M. A,, and Bradshaw, A. E. (1995) Investigation of haemostasis, in Practical Haematology (Dacie, J. V., and Lewis, S. M., eds). Churchill Livingstone, New York, pp. 297-315.
Mcintyre, B.A., Philp, R.B. and Inwood M.J. (2008). Effect of ibuprofen on platelet function in normal subjects and haemophiliac patients. Clin Pharmacal Ther. 24:616-621.
Ochei J, Kolhatkar A (2000). Medical Laboratory Science. Theory and Practice. Tata Mcgraw-Hill Publishing Company Limited: New Delhi. 2nd Edition, pp. 331-349.
Pal GK and Pal P (2001). Textbook of practical physiology, Orient Blackswan.
Ragni, M.V., Miller, B.J., Whalen, R. and Ptachcinski, R. (1992). Bleeding tendency, platelet function, and pharmacokinetics of ibuprofen and zidovudine in HIV(+) haemophilic men. Am 1 Hematol 1992;40: 176-182.
Saliu, J.A., Elekofehinti, O.O., Komolafe, K., Oboh, G. (2012). Effects of some green leafy vegetables on the Hematological parameters of Diabetic Rats. Scholars Research Library. J. Nat. Prod. Plant Resource. 2(4). 482 - 485
Simmons, D.L., Botting, R.M. and Hla, T. (2004). Cyclooxygenase Isozymes: The biology of prostaglandin synthesis and inhibition. Pharmacol Rev, 56: 387-437.
Starek, M., Krzek, J. (2009). A review of analytical techniques for determination of oxicams, nimesulide and nabumetone. Talanta. 77:925‑42.
Taivainen, T., Hiller, A., Rosenberg, P.H., and Neuvonen, P. (2009). The effect of continuous intravenous indomethacin infusion on bleeding time and postoperative pain in patients undergoing emergency surgery of the lower extremities. Acta Anaesthesia/ Scand. 33:58-60.
Taubert, KA. (2008). Can patients with cardiovascular disease take nonsteroidal anti-inflammatory drugs? Circul, 117: 322-324.
Wolfe DF. Urolithiasis. In: Wolfe DF and Moll HD, eds. Large Animal Urogenital Surgery. Philadelphia: Williams & Wilkins, 1999: 349-359.
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