Abstract
Background: Soil-transmitted helminth (STH)
infection remains a public health challenge.
Monitoring intensity of STH and associated
environmental factors are essential among school
age children (SAC) in developing countries. The study
determined the infection intensity of STH and
associated environmental factors among SAC in a
rural community in southeastern Nigeria.
Methods: A cross-sectional study was conducted on
221 SAC in a rural community in southeastern
Nigeria. The socio-demographic and Water,
Sanitation and Hygiene practices were collected with
a questionnaire and household sanitation facility and
pet ownership with observational checklist. Stool and
soil samples were collected and analyzed for STH
intensity. Data was analyzed using descriptive and
multiple regression analysis.
Results: A total of 221 SAC (9.3±2.5 years) provided
information on socio-demographics and 149 (67.4%)
gave stool samples. Hookworms (4.7%),
Strongyloides stercoralis (2.7%) and Enterobius
vermicularis (0.7%) were species identified.
Intensity of STHs expressed as eggs or larvae per
gram of stool (epg or lpg) showed light intensity for
Hookworms (37.5±18.8) and heavy for
Strongyloides stercoralis (25.2±0.1). Few (8.7%)
were infected with at least one helminth and 66.5%
SAC had recently dewormed (d”3 months). A
filariform larva of Strongyloides stercoralis was
found in a soil sample. About 61.5%, 75.0% and
60.0% participants indicated borehole as major source
of drinking water, presence of household toilet
facilities and pet ownership, respectively.
Conclusions: Soil-transmitted helminth intensity
varied in school-age children in this study. Soil
contamination with Strongyloides stercoralis larvae
implies repeated infections despite periodic
deworming. Environmental factors associated with
infections among school-age children in this rural
community should be addressed.
Referenser
WHO. Soil-transmitted helminthiases: eliminating
soil-transmitted helminthiases as a public health
problem in children: progress report 2001–2010
and strategic plan 2011–2020. Switzerland: World
Health Organization, 2012.
Al-Mekhlafi HM, Lim YAL and Ngui R. Soiltransmitted helminths: the neglected parasites.
In: Lim YAL, Vythilingam I, eds. Parasites and
their vectors. Springer-Verlag Wien, 2013: 205-
32.
World Health Organization. Prevention and control
of schistosomiasis and soil transmitted helminthiasis.
Report of a WHO Expert Committee. Geneva,
(WHO Technical Report Series, No 912), 2002
Uhuo AC, Nwanchor KC, Umene DA et al. Soil
transmitted parasites in fruits and vegetables planted
in landscape management nursery, College of
Agricultural Sciences, Ebonyi State University,
Abakaliki, Ebonyi State, implications to Public
Health. J. Biol. Med. Sci. 2015; 3: 5-7.
Horiuchi S, Paller VG and Uga S. Soil
contamination by parasite eggs in rural village in
the Philippines. Trop Biomed. 2013; 30:495–503.
Brooker S, Clements ACA and Bundy DAP.
Global epidemiology, ecology and control of soiltransmitted helminth infections. Adv. Parasitol.
: 62:221–61. doi:10.1016/S0065-
X(05)62007-6
Saathoff E, Olsen A, Magnussen P. et al. Patterns
of Schistosoma haematobium infection, impact of
praziquantel treatment and re-infection after
treatment in a cohort of schoolchildren from rural
KwaZulu-Natal/South Africa. BMC Infect Dis.
; 4(40): 1-10. https://doi.org/10.1186/1471-2334-
-40
Soil-transmitted Helminths and associated environmental factors 149
Pasaribu AP, Alam A, Sembiring K, Pasaribu S and
Setiabudi D. Prevalence and risk factors of soiltransmitted helminthiasis among school children
living in an agricultural area of North Sumatera,
Indonesia. BMC Public Health. 2019; 19(1066): 1-
https://doi.org/10.1186/s12889-019-7397-6
Olaniyi JE, Muktar HA and Pauline EJ. A review
of intestinal helminthiasis in Nigeria and the need
for school-based intervention. JRTPH. 2007; 6:
-39.
World Health Organization. Soil-transmitted
helminthiases Situation and trends. Global Health
Observatory (GHO) data, 2017 [Cited 2020 Dec
Available from: https://www.who.int/gho/
neglec te d_disea ses/soil_t ransmi tted_
helminthiases/en/
Anuar T, Salleh F and Moktar N. Soil-transmitted
helminth infections and associated risk factors
in three Orang Asli tribes in Peninsular Malaysia.
Sci Rep. 2014; 4, 4101. https://doi.org/10.1038/
srep04101
Awasthi S, Bundy DA and Savioli L. Helminthic
infections. BMJ. 2003; 327(7412): 431-433.
doi:10.1136/bmj.327.7412.431
Onwe IM and Omonona OV. Hydrochemical
characteristics and quality assessment of
groundwater from Umuogudu Oshia, Ngbo, S.E,
Nigeria. J. Nat. Sci. Res. 2016; 6(10): 31-7.
Charan J and Biswas T. How to calculate sample
size for different study designs in medical
research?. Indian J Psychol Med. 2013; 35:121-
Oluwole AS, Ekpo UF, Karagiannis-Voules DA,
et al. Bayesian geostatistical model-based
estimates of Soil-Transmitted Helminth infection
in Nigeria, including annual deworming
requirements. PLoS Negl. Trop. Dis. 2015; 9(4):
e0003740. doi:10.1371/journal.pntd.0003740
Cheesbrough M. District laboratory Practice in
tropical Countries, Part 1(2nd ed). Cambridge
University Press. 2005: 194-202.
World Health Organization. Preventive
chemotherapy in human helminthiasis.
Coordinated use of anthelminthic drugs in control
interventions: a manual for health professionals
and programme managers. Geneva, 2006
Khieu V, Schär F, Marti H, et al. Diagnosis,
treatment and risk factors of Strongyloides
stercoralis in schoolchildren in Cambodia. PLoS
Negl Trop Dis. 2013: 7(2):e2035. doi: 10.1371/
journal.pntd.0002035. PMID: 23409200;
PMCID: PMC3566990.
Schär F, Hattendorf J, Khieu V, Muth S, Char
MC, Marti HP, Odermatt P. Strongyloides
stercoralis larvae excretion patterns before and
after treatment. Parasitology. 2014: 141(7):892-
doi: 10.1017/S0031182013002345. PMID:
Forrer A, Khieu V, SchaÈr F, et al. Strongyloides
stercoralis is associated with significant
morbidity in rural Cambodia, including stunting
in children. PLoS Negl Trop Dis. 2017: 11(10):
e0005685. https://doi.org/10.1371/
journal.pntd.0005685
Kassa D, Mengestie A, Ayalew N, et al. A
review on diagnostic techniques in veterinary
helminthlogy. Nat Sci. 2016; 14(7):109-18.
Mclean EO. Soil pH and lime requirements. In:
Page AL (Ed.), Method of soil analysis. Part 2
Agronomy 9. (2nd edn), ASA and SSA Madison
Wisconsin, USA, 1982; 595-624.
Walkley A and Black IA. An examination of
Degtjareff Method for Determining Soil Organic
Matter and a proposed modification of the
chromic acid titration method. Soil Sci. 1934;
:29-37.
Odinaka KK, Nwolisa EC, Mbanefo F, Iheakaram
AC and Okolo S. Prevalence and Pattern of SoilTransmitted Helminthic Infection among Primary
School Children in a Rural Community in Imo State,
Nigeria. J. Trop. Med. 2015; Vol 2015; 1-4. http://
dx.doi.org/10.1155/2015/349439
Campbell SJ, Nery SV, Wardell R, et al. Water,
Sanitation and Hygiene (WASH) and
environmental risk factors for soil-transmitted
helminth intensity of infection in Timor-Leste,
using real time PCR. PLOS Negl. Trop. Dis.
; 11(3): e0005393. https://doi.org/10.1371/
journal.pntd.0005393
Omitola OO, Mogaji HO, Oluwole AS, et al.
Adeniran AA, Alabi OM, Ekpo UF. Geohelminth
Infections and Nutritional Status of Preschool
Aged Children in a Periurban Settlement of Ogun
State. Scientifica. 2016; Vol 2016: 1-9. Article
ID 7897351. https://doi.org/10.1155/2016/
Adeyeba OA and Akinlabi AM. Intestinal parasitic
infections among school children in a rural
community, Southwest Nigeria. Nig. J. Parasitol.
; 23:11–18.
Oyebamiji DA, Ebisike AN, Egede JO and Hassan
AA. Knowledge, attitude and practice with
respect to soil contamination by Soil-Transmitted
Helminths in Ibadan, Southwestern Nigeria,
Parasite Epidemiol. Control. 2018; 3 (4): e00075.
EO Ngwu, SJ Akinsete and M Adejumo
Debalke S, Worku A, Jahur N and Mekonnen Z.
Soil transmitted helminths and associated factors
among schoolchildren in government and private
primary school in Jimma Town, Southwest
Ethiopia. Ethiop J Health Sci. 2013; 23(3): 237-
doi: 10.4314/ejhs.v23i3.6.
Uneke U, Eze K, Oyibo P, Azu N and Ali E. Soiltransmitted helminth infection in school children
in South-Eastern Nigeria: The Public Health
implication. Internet J. Third World Med. 2006;
: 1-7.
Montresor A, Deol A, à Porta N, Lethanh N and
Jankovic D. Markov model predicts changes in
STH prevalence during control activities even
with a reduced amount of baseline information.
PLoS Negl Trop Dis. 2016; 10(4): e0004371.
https://doi.org/10.1371/journal.pntd.0004371
Emmy-Egbe IO. Faecal disposition methods and
incidence of intestinal helminth parasites among
school children in Ihiala Local Government Area,
Anambra State, Nigeria. Int. J. Sci. Res. 2013;
-87.
Kamalu NA, Uwakwe FE and Opara JA.
Prevalence of intestinal parasite among high
school students in Nigeria. Acad. J. Interdiscip.
Stud. 2013; 2(7) 9-16. doi: 10.5901/
ajis.2013.v2n7p9
Wosu M I and Onyeabor AI. The prevalence of
intestinal parasite infections among school
children in a tropical rainforest community of
Southeastern Nigeria. J. Anim. Sci. Adv. 2014;
(8):1004 - 8.
Adefioye OA, Efunshile AM, Ojurongbe O et
al. Intestinal Helminthiasis among School
Children in Ilie, Osun State, Southwest, Nigeria.
Sierra Leone J. Biomed. Res. 2011; 3(1):36-42.
Etewa SE, Abdel-Rahman SA, Abd El-Aal NF,
et al. Geohelminths distribution as affected by
soil properties, physicochemical factors and
climate in Sharkyia governorate Egypt. J Parasit
Dis. 2016; 40(2):496-504. doi: 10.1007/s12639-
-0532-5
Bethony J, Brooker S, Albonico M, et al. Soil
transmitted helminthic infections: ascariasis,
trichuriasis and hookworm. Lancet. 2006;
:1521–32. doi: 10.1016/S0140-
(06)68653-4
Landon JR. Brooker tropical soil manual: a
handbook for soil survey and agricultural land
evaluation in the tropics and sub-tropics. 1991;
pp 474
Ovutor O, Imafidor H and Awi-wada GDB.
Assessment of physic-chemical parameters of
soils in fallowing farmlands and pit toilet
environments as it affects the abundance of
geohelminths in Emohua Local Government
Area, Rivers State, Nigeria. Annu. Res. Rev.
Biol. 2017; 14(3):1-10. https://doi.org/10.9734/
ARRB/2017/31546
Nwoke EU, Odikamnoro OO, Ibiam GA, Umah
OV and Ariom OT. A survey of common gut
helminth of goats slaughtered at Ankpa abattoir,
Kogi State, Nigeria. J. Parasitol. Vector Biol.
; 7(5):89-93. doi: 10.5897/JPVB2015.0195
Forrer A, Khieu V, Vounatsou P, et al.
Strongyloides stercoralis: Spatial distribution of
a highly prevalent and ubiquitous soil-transmitted
helminth in Cambodia. PLoS Negl Trop Dis.
; 13(6): e0006943. https://doi.org/10.1371/
journal.pntd.0006943
Umar AA and Bassey SE. Incidence
of Strongyloides stercoralis infection in Ungogo,
Nassarawa, Dala and Fagge Local Government
Areas of Kano state, Nigeria. BAJOPAS 2010;
(2):76–80. doi: 10.4314/bajopas.v3i2.63224