Resumo
Background: Water-borne and food-borne
outbreaks of diseases triggered by Plesiomonas
shigelloides have been reported in many parts of
the world. Water has always been the major source
or route of contaminant implicated in many disease
outbreaks.
Methodology: We conducted this study to establish
the prevalence and genetic variability of P.
shigelloides from fish, vegetable, and water sources
from Southwest Nigeria. Fish, vegetables, pond water,
irrigation water, and surface water samples were
collected from Southwest Nigeria and cultured on
Inositol brilliant green bile salt agar after enrichment
procedure. Simplex PCR was used to screen P.
shigelloides isolates using 23SrRNA primers with
the expected band size of 628bp. The confirmed
isolates were further characterised by RAPD typing
using OPA primers and RAPD patterns
analysed with NTSYS software.
Results: A total of 36 isolates were confirmed
positive for P. shigelloides. OPA 4 was the best
primers for P. shigelloides detection producing the
best polymorphic bands. Genetic variability was
observed among the P. shigelloides strains. The
results obtained indicated that irrigation, pond, and
surface water may be the transmission route of P.
shigelloides as they are closely related to strains
obtained from vegetables and fishes.
Conclusion: Since fish and vegetable farming rely
majorly on surface water such as rivers for irrigation
and pond water supply, P. shigelloides from surface
water may pose serious infection risk compared to
other environmental sources in this region.
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