Synergistic Enhancement of Motor Function by Curcumin and Piperine in Alpha-Synuclein Expressing Drosophila Models
DOI:
https://doi.org/10.47081/njn2024.15.2/004Keywords:
α-synuclein, Curcumin, Climbing activity, Drosophila melanogaster, Parkinson disease, PiperineAbstract
Parkinson’s disease (PD) is a neurodegenerative disease characterized by Lewy body formation mainly phosphorylated and aggregated α-synuclein, with subsequent neurotoxicity and death of dopaminergic neurons in the basal ganglia. The neuroprotective action of curcumin and piperine has been reported. We used the GAL4/UAS bipartite system for targeted expression of α-synuclein pan-neuronal and dopamine neurons to create genetically induced PD. Hence, in this study we assessed the ameliorative effect of curcumin and piperine on α-synuclein-induced dopaminergic neuron degeneration and photoreceptor degeneration in the Drosophila melanogaster model of PD. Male Drosophila melanogaster (upstream activating sequence (UAS) linked to synuclein (SNCA) flies were crossed with virgin wild-type Canton-S, DDC-GAL4 or GMR-GAL4 female flies) cultured on either medium containing graded concentrations of curcumin and/or piperine (5, 10, and 50 μM) or vehicle. The parameters measured included fecundity, larval motility, negative geotaxis, and lifespan. Vehicle, curcumin, or piperine supplementation in SNCA>Cs, SNCA>DDC, or SNCA>GMR flies did not affect fecundity or larval motility, but piperine (5 μM and 10 μM but not 50 μM) supplementation reduced fecundity. SNCA>DDC flies significantly reduced climbing performance and lifespan, peaked in week 4, ameliorated by the curcumin and piperine combination. Moreover, the increased aggregation of synuclein, as evidenced in ommatidial degeneration in SNCA>GMR flies’ eyes, was attenuated by the curcumin and piperine combination. Findings from the present study further reinforced the potential benefit of curcumin and piperine in the management of PD.
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