Spondias mombin L. Fruit Pulp Prevents D-Galactose-Induced Cognitive Deficits, Oxidative Stress and Neurodegeneration in Wistar Rats' Prefrontal Cortex
DOI:
https://doi.org/10.47081/njn2025.16.2/002Keywords:
Spondias mombin, Acetylcholinesterase activities, Ageing, Oxidative stressAbstract
D-galactose (D-Gal) administration is known to cause cognitive impairment in animal models of ageing. Spondias mombin L. fruit pulp (SM) is known to ameliorate stress-induced conditions caused by oxidative stress. The neuroprotective role of SM against D-Gal-induced stress in Wistar rats was investigated. Forty-two male Wistar rats were divided into six groups (n=7): control group were fed with normal diet; D-Gal group were treated with D-Gal only; Gal + SM5 group were fed with 5% SM (w/w)-containing diet and treated with D-Gal; Gal + SM10 group were fed with 10% SM (w/w)-containing diet and treated with D-Gal; SM 5 group were fed with 5% SM-containing diet; and SM 10 group were fed with 10% SM-containing diet. Control, SM5, and SM10 groups were treated by 0.9% sodium chloride/day (subcutaneous (s.c), 5 days per week) while D-Gal treatment was 250 mg/kg of D-Gal (s.c., 5 days per week). Administration was done for 6 weeks after which behavioural assessments were conducted, followed by biochemical and histopathological analyses of the prefrontal cortex. The administration of SM-containing diet at the two concentrations investigated in this study showed improvements in cognitive impairment caused by the D-Gal compared to the control. Biochemical assays indicated that D-Gal significantly disrupted antioxidant status, proinflammatory and anti-inflammatory cytokines. SM supplementation reversed these effects. Histopathological analysis confirmed that SM5 and SM10 significantly reversed D-Gal-induced cortical alterations. In conclusion, SM attenuated D-Gal-induced cognitive deficits, oxidative stress, and neurodegeneration in Wistar rats.
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Ahmad, S., Khan, A., Ali, W., Jo, M. H., Park, J., Ikram, M., et al. (2021). Fisetin rescues the mice brains against D-Galactose-induced oxidative stress, neuroinflammation and memory impairment. Front Pharmacol 12, 612078. doi: 10.3389/fphar.2021.612078
Aon-Im, P., Monthakantirat, O., Daodee, S., Chulikhit, Y., Sriya, N., Boonyarat, C., et al. (2024). Evaluation of the impact of Alternanthera philoxeroides (Mart.) Griseb. Extract on memory impairment in D-galactose-induced brain aging in mice through its effects on antioxidant enzymes, neuroin-flammation, and telomere shortening. Molecules 29(2), 503. doi: 10.3390/molecules29020503
Azman, K. F., and Zakaria, R. (2019). D-Galactose-induced accelerated ageing model: an overview. Biogerontology 20(6), 763-782. doi:.10.1007/s10522-019-09837-y
Buccellato, F. R., D'Anca, M., Fenoglio, C., Scarpini, E., and Galimberti, D. (2021). Role of oxidative damage in alzheimer's disease and neurodegeneration: From pathogenic mechanisms to biomarker discovery. Antioxidants 10(9), 1353. doi: 10.3390/antiox10091353
Cabral, B., Siqueira, E. M., Bitencourt, M. A., Lima, M. C., Lima, A. K., Ortmann, C. F., et al. (2016). Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves. Revista Brasileira de Farmacognosia 26, 304-311. doi: 10.1016/j.bjp.2016.02.002
de Souza, V. R., Lima, T. P. B., Bedê, T. P., Faria, S. B. A., Alves, R., Louzada, A., et al. (2023). Murici (Byrsonima crassifolia (L.) Kunth and verbascifolia (L.)) and tapereba (Spondias mombin) improve hepatic and inflammatory biomarkers in high-fat-diet rats. Foods 12(2), 255. doi: 10.3390/foods12020255
Dharmarajan, T. (2021). Physiology of ageing. Geriatric Gastroenterology 101-153. doi: 10.1007/978-3-030-30192-7_5
Ellman, G. L. (1959). Tissue sulfhydryl groups. Arch Biochem Biophys 82(1), 70-77. doi: 10.1016/0003-9861(59)90090-6
Ellman, G. L., Courtney, K. D., Andres Jr, V., and Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7(2), 88-95. doi: 10.1016/0006-2952(61)90145-9
Ennaceur, A. (2010). One-trial object recognition in rats and mice: methodological and theoretical issues. Behav Brain Res 215(2), 244-254. doi: 10.1016/j.bbr.2009.12.036
Evuen, U., and Kpomah, E. D. (2023). Comparative impact of solvent extracts of Spondias mombin leaves on in-vitro antioxidant and acetylcholinesterase inhibitory activities (2023). Asian J Res Biochem 12(3), 20-29. DOI: 10.9734/ajrb/2023/v12i3236
Fuster, J. (2017). Prefrontal cortex in decision-making: The perception-action cycle. Decision neuroscience. Elsevier, pp. 95-105. doi: 10.1016/B978-0-12-805308-9.00008-7
Habig, W. H., Pabst, M. J., and Jakoby, W. B. (1974). Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 249(22), 7130-7139. doi: 10.1016/S0021-9258(19)42083-8
Izquierdo, I., and Dias, R. D. (1983). Effect of ACTH, epinephrine, β-endorphin, naloxone, and of the combination of naloxone or β-endorphin with ACTH or epinephrine on memory consolidation. Psychoneuroendocrinology, 8(1), 81-87. doi: 10.1016/0306-4530(83)90043-4
Jeje, S. O., Omayone, T. P., Oyeleye, I. S., Adegoke, A. M., and Adeagbo, A. S. (2024) Protective role of vitamin supplementation against maternal stress-induced altera-tions in cardiovascular indices and cardiac oxidative stress in offspring of Wistar rats. FUTA Journal of Life Sciences 4(1), 39-52.
Kumar, H., Bhardwaj, K., Valko, M., Alomar, S. Y., Alwasel, S. H., Cruz-Martins, N., Dhanjal, D. S., Singh, R., Kuča, K., and Verma, R. (2022). Antioxidative potential of Lactobacillus sp. in ameliorating D-Galactose-induced age-ing. Appl Microbiol Biotechnol 106(13), 4831-4843. doi: 10.1007/s00253-022-12041-7
Kuo, H.-C., Tong, S.-Y., Chao, M.-W., and Tseng, C.-Y. (2022). Ganoderma tsugae prevents cognitive impairment and attenuates oxidative damage in D-Galactose-induced ageing in the rat brain. PLoS One 17(4), e0266331. doi: 10.1371/journal.pone.0266331
Magrone, T., Magrone, M., Russo, M. A., and Jirillo, E. (2019). Recent advances on the anti-inflammatory and antioxidant properties of red grape polyphenols: in vitro and in vivo studies. Antioxidants 9(1), 35. doi: 10.3390/antiox9010035
McCord, J. M., and Fridovich, I. (1969). Superoxide dis-mutase: an enzymic function for erythrocuprein (hemocu-prein). J boil Chem 244(22), 6049-6055. doi: 10.1016/S0021-9258(18)63504-5
Moke, E. G., Edje, E. K., Daubry, T. M., Nwogueze, B. C., Ataikiru, O. M., Umukoro, E. K., et al. (2024). Phyto-pharmacological Activities of Spondias mombinLinn: A Re-view: Tropical Journal of Phytochemistry and Pharmaceutical Sciences 3(1), 117-123. doi:10.26538/tjpps/v3i1.1
Morrison, J. H., and Baxter, M. G. (2012). The ageing cortical synapse: hallmarks and implications for cognitive decline. Nat Rev Neurosci 13(4), 240-250. doi: 10.1038/nrn3200
Mukherjee, P. K., Kumar, V., and Houghton, P. J. (2007). Screening of Indian medicinal plants for acetylcholinesterase inhibitory activity. Phytother Res 21(12), 1142-1145. doi: 10.1002/ptr.2224
Mursal, M., Kumar, A., Hasan, S. M., Hussain, S., Singh, K., Kushwaha, S. P., et al (2024). Role of natural bioactive compounds in the management of neurodegenerative disorders. Intelligent Pharmacy 2(1), 102-113. doi: 10.1016/j.ipha.2023.09.006
Ohkawa, H., Ohishi, N., and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2), 351-358. doi: 10.1016/0003-2697(79)90738-3
Osuntokun, O. S., Aderoju, M. O., Adebisi, I. E., Abayomi, T. A., Tokunbo, O. S., and Olayiwola, G. (2021). Phenytoin-levetiracetam adjunctive treatment-induced neurotoxicity and deregulation of cholinergic neurotransmission with evidence of neurocognitive impairment in male Wistar rats. Alex J Med 57(1), 194-204. doi: 10.1080/20905068.2021. 1948157
Rossato, D. R., Rosa, J. L., Fontoura, M. B., de Souza, L. E., de Almeida, T. M., Kudrna, K. B., et al. (2024). Ferulic acid-loaded nanostructure maintains brain levels of ACh, glutamate, and GABA and ameliorates anxiety and memory impairments induced by the D-galactose ageing process in rats. Neurochem Res, 49(12), 3383-3395. doi: 10.1007/s11064-024-04248-z
Samad, N., Hafeez, F., and Imran, I. (2022). D-Galactose induced dysfunction in the mice hippocampus and the possible antioxidant and neuromodulatory effects of selenium. Environmental Science and Pollution Research 29(4), 5718-5735. doi: 10.1007/s11356-021-16048-x
Seibenhener, M. L., and Wooten, M. C. (2015). Use of the open field maze to measure locomotor and anxiety-like behavior in mice. J Vis Exp (96), 52434. doi: 10.3791/52434-v
Sharma, C., Kim, S., Nam, Y., Jung, U. J., and Kim, S. R. (2021). Mitochondrial dysfunction as a driver of cognitive impairment in Alzheimer's disease. Int J Mol Sci 22(9), 4850. doi: 10.3390/ijms22094850
Siswanto, F. M., Wijaya, I. M. T., Handayani, M. D. N., Dewi, R., Ekowati, A. L., and Manalu, J. L. (2024). Extract of Angelica keiskei Leaves Attenuates Spatial Memory Impairment on the D-g model of brain ageing in mice. Biomed Pharmacol J 17(3), 1563-1573. doi: 10.13005/bpj/2965
Vuolo, M. M., da Silva-Maia, J. K., and Batista, Â. G. (2022). The GSH colorimetric method as measurement of antioxidant status in serum and rodent tissues. In Basic Protocols in Foods and Nutrition. Springer. pp. 187-194. doi: 10.1007/978-1-0716-2345-9_12
Wang, K., Liu, H., Hu, Q., Wang, L., Liu, J., Zheng, Z., et al.. (2022). Epigenetic regulation of ageing: implications for interventions of ageing and diseases. Signal Transduct Target Ther 7(1), 374. doi: 10.1038/s41392-021-00773-3
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